AAV_hSyn_Ilmol
(Plasmid
#120311)
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PurposeExpresssion of Ilmol preferentially in neurons
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 120311 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4500
- Total vector size (bp) 6000
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIlmol
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Alt nameIlmol
- Promoter hSyn
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer CCC AAG CTG GCT AGC GCC ACC ATG GAG GGA TTC GAC
- 3′ sequencing primer GAT AAG CTT GAT ATC GAA TTC TCA CTT GTA CAG CTC GTC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV_hSyn_Ilmol was a gift from Bradley Baker (Addgene plasmid # 120311 ; http://n2t.net/addgene:120311 ; RRID:Addgene_120311) -
For your References section:
A dimeric fluorescent protein yields a bright, red-shifted GEVI capable of population signals in brain slice. Yi B, Kang BE, Lee S, Braubach S, Baker BJ. Sci Rep. 2018 Oct 12;8(1):15199. doi: 10.1038/s41598-018-33297-y. 10.1038/s41598-018-33297-y [pii] PubMed 30315245
Map uploaded by the depositor.
