PurposeBarcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||120426||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9514
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)708
- Promoter EF1a
- Cloning method Gibson Cloning
- 5′ sequencing primer 5'-ttctcaagcctcagacagtgg-3' (Common Sequencing Primers)
5' cloning site: BamHI (not destroyed), 3' cloning site: BamHI (not destroyed).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EF1a_mCherry_P2A_Hygro_Barcode was a gift from Prashant Mali (Addgene plasmid # 120426 ; http://n2t.net/addgene:120426 ; RRID:Addgene_120426)
For your References section:Mapping Cellular Reprogramming via Pooled Overexpression Screens with Paired Fitness and Single-Cell RNA-Sequencing Readout. Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P. Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 10.1016/j.cels.2018.10.008. 10.1016/j.cels.2018.10.008 PubMed 30448000