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35S:5mARF17 pBINPLUS
(Plasmid #12075)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12075 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pBINPLUS
  • Backbone size w/o insert (bp) 12300
  • Vector type
    plant transformation vector
  • Selectable markers
    Plant Kanamycin Resistance

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5mARF17
  • Alt name
    auxin response transcription factor 17
  • Alt name
    ARF17
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    3300
  • Mutation
    miR160-resistant version of ARF17 has five silent mutations within the miR160-complementary domain of an ARF17 genomic clone, thereby increasing the number of mismatches between miR160 and the ARF17 mRNA from one in wild type to six.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer 35Spromoter (5'-CTA TCC TTC GCA AGA CCC TTC-3')
  • 3′ sequencing primer CaMV term rev (5'-TTG ATA GAA GTA TTT TAC AAA TAC-3')
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A 35S:5mARF17 fragment was subcloned into the binary vector pBINPLUS. See "sequence" for full insert sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    35S:5mARF17 pBINPLUS was a gift from David Bartel (Addgene plasmid # 12075)

  • For your References section:

    MicroRNA-directed regulation of Arabidopsis AUXIN RESPONSE FACTOR17 is essential for proper development and modulates expression of early auxin response genes. Mallory AC, Bartel DP, Bartel B. Plant Cell. 2005 May . 17(5):1360-75. 10.1105/tpc.105.031716 PubMed 15829600
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