Addgene - pSico p53 Plasmid Data

Plasmid 12089: pSico p53

Gene/insert name:
p53 shRNA
Alt name:
p53
Insert size:
55
Species:
M. musculus (mouse)
Entrez Gene:
Trp53 (RP23-56I20.1, Tp53, bbl, bfy, bhy, p44, p53)
Mutation:
EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. Cre causes EGFP to be recombined out of the construct, activating p53 shRNA expression.
Vector backbone:
pSico
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size w/o insert (bp):
7560
Cloning site 5':
HpaI
Site destroyed during cloning:
Yes
Cloning site 3':
XhoI
Site destroyed during cloning:
Yes
5' sequencing primer:
EGFP-C List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

Cre TURNS ON p53 shRNA expression. The shRNA is directed against the mouse p53 sequence 5'-GTACTCTCCTCCCCTCAAT-3'.

Follow safety guidelines when handling live virus expressing p53 shRNA.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cre-lox-regulated conditional RNA interference from transgenes. Ventura et al (Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12089" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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