Addgene - pSicoR p53 Plasmid Data

Plasmid 12090: pSicoR p53

Gene/insert name:
p53 shRNA
Alt name:
p53
Insert size:
55
Species:
M. musculus (mouse)
Entrez Gene:
Trp53 (RP23-56I20.1, Tp53, bbl, bfy, bhy, p44, p53)
Mutation:
EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off p53 shRNA expression.
Vector backbone:
pSicoR
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size w/o insert (bp):
7560
Cloning site 5':
HpaI
Site destroyed during cloning:
Yes
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
mU6-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

Cre TURNS OFF p53 shRNA expression. The shRNA is directed against the mouse p53 sequence 5'-GTACTCTCCTCCCCTCAAT-3'.

Follow safety guidelines when handling live virus expressing p53 shRNA.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cre-lox-regulated conditional RNA interference from transgenes. Ventura et al (Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12090" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

12089: pSico p53

11579: pSicoR

11578: pSico

12084: pSicoR PGK puro

9058: pBABE-hygro p53 DD