|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12108||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6670
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberLow Copy
Insert Size (bp)3221
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LV-Lac was a gift from Inder Verma (Addgene plasmid # 12108 ; http://n2t.net/addgene:12108 ; RRID:Addgene_12108)
For your References section:Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivo. Pfeifer A, Brandon EP, Kootstra N, Gage FH, Verma IM. Proc Natl Acad Sci U S A. 2001 Sep 25. 98(20):11450-5. 10.1073/pnas.201415498 PubMed 11553794