Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12116||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepGEM-T Easy
- Backbone size w/o insert (bp) 3015
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemU6 promoter
Insert Size (bp)707
- Cloning method Restriction Enzyme
- 5′ cloning site PCR (unknown if destroyed)
- 3′ cloning site PCR (unknown if destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PGTE+mU6-WT was a gift from Inder Verma (Addgene plasmid # 12116 ; http://n2t.net/addgene:12116 ; RRID:Addgene_12116)
For your References section:CRE recombinase-inducible RNA interference mediated by lentiviral vectors. Tiscornia G, Tergaonkar V, Galimi F, Verma IM. Proc Natl Acad Sci U S A. 2004 May 11. 101(19):7347-51. 10.1073/pnas.0402107101 PubMed 15123829