pPTK
(Plasmid
#12125)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12125 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonen/a
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHIV delta R 8.2
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Alt nameall HIV-1 genes except envelope
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Insert Size (bp)7800
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglI (not destroyed)
- 3′ cloning site SacII (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The pPTK plasmid contains an HIV-1 genomic fragment that encodes all HIV-1 genes excluding the HIV-1 envelope (R.8.2) under the regulation of an inducible promoter. This promoter contains the TRE and the human CMV minimal promoter (pHCMV).
Please note that there is more than one BglI site in this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPTK was a gift from Inder Verma (Addgene plasmid # 12125) -
For your References section:
A packaging cell line for lentivirus vectors. Kafri T, van Praag H, Ouyang L, Gage FH, Verma IM. J Virol. 1999 Jan . 73(1):576-84. PubMed 9847362
Map uploaded by the depositor.