Addgene - FLAG-Foxo1ADA(pCMV5) Plasmid Data

Plasmid 12149: FLAG-Foxo1ADA(pCMV5)

Gene/insert name:
Foxo1 ADA
Alt name:
Fkhr ADA
Alt name:
Foxo1
Alt name:
Fkhr
Insert size:
1700
Species:
M. musculus (mouse)
GenBank ID:
NM_019739
Entrez Gene:
Foxo1 (AI876417, Afxh, FKHR, Fkhr1, Foxo1a)
Fusion protein or tag:
FLAG
Terminal:
N terminal on insert
Mutation:
Threonine 24 to Alanine (A) and Serine 253 to Asparete (D) and Serine 316 to Alanine (A)
Vector backbone:
pCMV5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CGTTGACGCAAATGGGCGG List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Domenico Accili
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: FoxO1 protects against pancreatic beta cell failure through NeuroD and MafA induction. Kitamura et al (Cell Metab. 2005 Sep . 2(3):153-63. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12149" in your Materials and Methods section.