|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12158||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerVerma Lab
- Backbone size (bp) 7140
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
pCS has a 133-bp deletion in the 3' LTR, generating a self-inactivating (SIN) virus. See article for cloning details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS was a gift from Inder Verma (Addgene plasmid # 12158 ; http://n2t.net/addgene:12158 ; RRID:Addgene_12158)
For your References section:Development of a self-inactivating lentivirus vector. Miyoshi H, Blomer U, Takahashi M, Gage FH, Verma IM. J Virol. 1998 Oct . 72(10):8150-7. PubMed 9733856