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pBAD-BirA-eCPX-AP(K10)
(Plasmid #121908)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 121908 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBAD
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5919
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    BirA
  • Species
    Synthetic
  • Insert Size (bp)
    984
  • Entrez Gene
    birA (a.k.a. b3973, ECK3965, bioR, dhbB)
  • Promoter pBAD promoter
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    enhanced Circularly Permuted Outer Membrane Protein X
  • Alt name
    eCPX
  • Species
    Synthetic
  • Insert Size (bp)
    627
  • Mutation
    changed lysine 10 in the AP to alanine
  • Promoter T7

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-BirA-eCPX-AP(K10) was a gift from Per Svenningsen (Addgene plasmid # 121908 ; http://n2t.net/addgene:121908 ; RRID:Addgene_121908)
  • For your References section:

    A bacterial display system for effective selection of protein-biotin ligase BirA variants with novel peptide specificity. Granhoj J, Dimke H, Svenningsen P. Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.1038/s41598-019-40984-x. 10.1038/s41598-019-40984-x PubMed 30858523