Brg1 Del1 pET28a
(Plasmid #122115)

• Purpose: Expression Brg1 amino acids 1-381 in bacteria
• Depositing Lab: Paul Herring
• Publication: Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;29(6):921-8. doi: 10.1161/ATVBAHA.109.187229. Epub 2009 Apr 2.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Brg1
• Alt name: SMARCA4
• Species: H. sapiens (human)
• Insert Size (bp): 1142
• Entrez Gene: SMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)
• Tag / Fusion Protein:
  • His-T7 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Supplemental Documents:
  • Brg1 Del1 pET28a seq

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry