PurposeExpresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||122442||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9000
- Total vector size (bp) 12142
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameHP1 alpha
SpeciesH. sapiens (human)
Insert Size (bp)570
Entrez GeneCBX5 (a.k.a. HEL25, HP1, HP1A)
- Promoter SFFV
/ Fusion Proteins
- miRFP670 (C terminal on insert)
- Cry2WT (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byHP1 alpha was subcloned from Addgene 17652 (Cheutin et al., Science, 2003). miRFP670 was from Addgene plasmid #79987 (Shcherbakova et al., Nat. Commun., 2016).
Terms and Licenses
Our Cry2WT contains an additional 29 amino acids at the C-terminus of Cry2PHR the same as our previously deposited plasmid Addgene #101223 (Shin et al., Cell, 2017).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHR-HP1α-miRFP670-Cry2WT was a gift from Clifford Brangwynne (Addgene plasmid # 122442 ; http://n2t.net/addgene:122442 ; RRID:Addgene_122442)
For your References section:Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen NS, Haataja M, Brangwynne CP. Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057. 10.1016/j.cell.2018.10.057 PubMed 30500535