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pHR-HP1α-miRFP670-Cry2WT
(Plasmid #122442)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 122442 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHR
  • Backbone size w/o insert (bp) 9000
  • Total vector size (bp) 12142
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HP1 alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    570
  • Entrez Gene
    CBX5 (a.k.a. HEL25, HP1, HP1A)
  • Promoter SFFV
  • Tags / Fusion Proteins
    • miRFP670 (C terminal on insert)
    • Cry2WT (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    HP1 alpha was subcloned from Addgene 17652 (Cheutin et al., Science, 2003). miRFP670 was from Addgene plasmid #79987 (Shcherbakova et al., Nat. Commun., 2016).
  • Terms and Licenses

Depositor Comments

Our Cry2WT contains an additional 29 amino acids at the C-terminus of Cry2PHR the same as our previously deposited plasmid Addgene #101223 (Shin et al., Cell, 2017).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-HP1α-miRFP670-Cry2WT was a gift from Clifford Brangwynne (Addgene plasmid # 122442 ; http://n2t.net/addgene:122442 ; RRID:Addgene_122442)
  • For your References section:

    Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen NS, Haataja M, Brangwynne CP. Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057. 10.1016/j.cell.2018.10.057 PubMed 30500535