pLKO.1 puro CXCR4 siRNA-2
(Plasmid #12272)

• Depositing Lab: Bob Weinberg
• Publication: Orimo et al Cell. 2005 May 6. 121(3):335-48.

Backbone

• Vector backbone: pLKO.1
• Backbone size w/o insert (bp): 7139
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CXCR4 siRNA
• Alt name: CXCR4
• Species: H. sapiens (human)
• Insert Size (bp): 59
• Entrez Gene: CXCR4 (a.k.a. CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS, WHIMS1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: LKO.1 5'

Resource Information

• Reference:
  • Addgene's pLKO.1 protocol
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CXCR4-siRNA-2 sequence: 5'-GTTTTCACTCCAGCTAACACA-3'