pBT100-neo
(Plasmid #123137)

• Purpose: Golden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.
• Depositing Lab: David Liu
• Publication: Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi: 10.1038/s41467-019-09987-0.

Backbone

• Vector backbone: pBT100
• Backbone size w/o insert (bp): 1700
• Total vector size (bp): 3000
• Vector type: Synthetic Biology ; Golden Gate donor vector
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Neomycin, poly-A terminator
• Promoter: none

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GCCTTTGAGTGAGCTGATACCG
• 3′ sequencing primer: GAAAAGTGCCACCTAAATTGTAAGCG

Resource Information

• Supplemental Documents:
  • pBT100.neo-cassette.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Golden gate donor vector which, in conjunction with pCALNL-EGFP-BsaI acceptor vector and two dsDNA recombinase targets, assembles to form a fluorescent EGFP transfectable reporter. Donor vector contains neomycin resistance cassette and poly-A terminator.