pSB90 - pAGM4723_VirGN54D
(Plasmid #123187)

• Purpose: (Empty Backbone) Golden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in Agrobacteria
• Depositing Labs: Erin Cram, Carolyn Lee-Parsons
• Publication: Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. eCollection 2019.

Backbone

• Vector backbone: pAGM4723
• Backbone manufacturer: Weber et al., 2011 (DOI:10.1371/journal.pone.0016765)
• Backbone size (bp): 12773
• Vector type: Plant Expression ; Level 2 acceptor vector for expression in plants
• Selectable markers: CRED

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VirGN54D from pAD1289 (a gift from A. Das)
• Species: Agrobacterium tumefaciens
• Insert Size (bp): 1153
• Mutation: VirGN54D for increase virulence

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pSB90 - pAGM4723_VirGN54D.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Golden Gate (MoClo; Weber et al., 2011) level 2 acceptor vector with Mutation VirGN54D for increased virulence. Increased virulence in Agrobacteria for a high-efficiency gene transfer (increased with and without acetosyringone, compared to original vector control).