pSPneoSagRNAH
(Plasmid
#123265)
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Purpose(Empty Backbone) Expresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 123265 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSPneo
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Vector typeCRISPR ; Leishmania Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 promoter (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Feng Zhang Addgene plasmid pX601
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSPneoSagRNAH was a gift from Greg Matlashewski (Addgene plasmid # 123265 ; http://n2t.net/addgene:123265 ; RRID:Addgene_123265) -
For your References section:
Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in Leishmania. Zhang WW, Matlashewski G. mSphere. 2019 Aug 21;4(4). pii: 4/4/e00408-19. doi: 10.1128/mSphere.00408-19. 10.1128/mSphere.00408-19 PubMed 31434745
