|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||123285||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4927
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)285
- Promoter CMV
/ Fusion Protein
- HaloTag7 (N terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The backbone for this construct is Addgene Plasmid #54249; mEmerald-Sec61-C-18 from the Michael Davidson Fluorescent Protein Collection. The mEmerald sequence was replaced with HaloTag7 complete sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Halo-Sec61-C-18 was a gift from Kevin McGowan (Addgene plasmid # 123285 ; http://n2t.net/addgene:123285 ; RRID:Addgene_123285)