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Halo-Sec61-C-18
(Plasmid #123285)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 123285 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Halo-C1
  • Backbone size w/o insert (bp) 4927
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sec61
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    285
  • GenBank ID
    NM_006808.2
  • Entrez Gene
    SEC61B
  • Promoter CMV
  • Tag / Fusion Protein
    • HaloTag7 (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The backbone for this construct is Addgene Plasmid #54249; mEmerald-Sec61-C-18 from the Michael Davidson Fluorescent Protein Collection. The mEmerald sequence was replaced with HaloTag7 complete sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Halo-Sec61-C-18 was a gift from Kevin McGowan (Addgene plasmid # 123285 ; http://n2t.net/addgene:123285 ; RRID:Addgene_123285)