Halo-Sec61-C-18
(Plasmid #123285)

• Purpose: Localization: Endoplasmic Reticulum
• Depositing Lab: Kevin McGowan
• Publication: Davidson HaloTag constructs (unpublished)

Backbone

• Vector backbone: Halo-C1
• Backbone size w/o insert (bp): 4927
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sec61
• Species: H. sapiens (human)
• Insert Size (bp): 285
• GenBank ID: NM_006808.2
• Entrez Gene: SEC61B
• Promoter: CMV
• Tag / Fusion Protein:
  • HaloTag7 (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • Halo-Sec61-C-18 SeqVerified.gbk
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The backbone for this construct is Addgene Plasmid #54249; mEmerald-Sec61-C-18 from the Michael Davidson Fluorescent Protein Collection. The mEmerald sequence was replaced with HaloTag7 complete sequence.