pLC-Flag-CSNK1A1 G40N-P2A-Blast
(Plasmid #123321)

• Purpose: Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Blast casette from a CMV promoter
• Depositing Lab: Eva Gottwein
• Publication: Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 10.1182/blood-2018-01-828418. Epub 2018 Jun 28.

Backbone

• Vector backbone: pLC-ZsGreen-P2A-Blast
• Total vector size (bp): 7954
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CSNK1A1
• Alt name: CK1 alpha
• Species: H. sapiens (human)
• Insert Size (bp): 1035
• Mutation: changed Glycine 40 to Asparagine
• Entrez Gene: CSNK1A1 (a.k.a. CK1, CK1a, CKIa, HEL-S-77p, HLCDGP1, PRO2975)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • pLC-Flag-CSNK1A1-G40N-P2A-Blast.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry