Addgene - pCL-MFG-BRCA1 Plasmid Data

Plasmid 12341: pCL-MFG-BRCA1

Gene/insert name:
BRCA1
Species:
H. sapiens (human)
Entrez Gene:
BRCA1 (BRCAI, BRCC1, BROVCA1, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
Vector backbone:
pCLMFG
(Search Vector Database)
Backbone manufacturer:
Verma Lab
Vector type:
Mammalian Expression, Retroviral
Cloning site 5':
NcoI (partial digest)
Site destroyed during cloning:
No
Cloning site 3':
XbaI/BamHI
Site destroyed during cloning:
Yes
5' sequencing primer:
LXSN primer List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Inder Verma
Terms and Licenses:
MTA

Comments:

Full-length BRCA1 cDNA was lifted from this construct as a NcoI (partial digest)-XbaI/blunted fragment and inserted into NcoI (partial digest)-cut and BamHI-cut/blunted pCL-MFG retroviral vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: BRCA1 is a cell cycle-regulated nuclear phosphoprotein. Ruffner et al (Proc Natl Acad Sci U S A. 1997 Jul 8. 94(14):7138-43. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12341" in your Materials and Methods section.