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pCLXSN
(Plasmid #12343)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12343 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCLXSN
  • Backbone manufacturer
    Verma Lab
  • Backbone size (bp) 7807
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A 3.1-kb fragment of LXSN, spanning from the SacI site in the 5'LTR near the U3-R junction to the AccI site just beyond the 3'LTR, was cloned into the 4.5-kb CMV expression construct, CMX-low, at a corresponding SacI site within the CMV immediate early enhancer-promoter.

NOTE: The depositor map indicated an EcoRI site present in this plasmid, but that site is ablated by a small insert.

Note that the full sequence and subsequent map are assembled and have not been verified by full sequencing. Please take this into account when submitting this plasmid to diagnostic digests.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLXSN was a gift from Inder Verma (Addgene plasmid # 12343 ; http://n2t.net/addgene:12343 ; RRID:Addgene_12343)
  • For your References section:

    The pCL vector system: rapid production of helper-free, high-titer, recombinant retroviruses. Naviaux RK, Costanzi E, Haas M, Verma IM. J Virol. 1996 Aug . 70(8):5701-5. PubMed 8764092