Gene/insert name: VCP R323K
Alt name: p97
Insert size: 2400
Species:
M. musculus (mouse)
Entrez Gene:
Vcp
()
Fusion protein or tag: His6
Terminal: N terminal on backbone
Mutation: R323K
Vector backbone:
pET-28aSearch Vector Database )
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
NdeI
Site destroyed during cloning:
Unknown
Cloning site 3':
BamH1
Site destroyed during cloning:
Unknown
5' sequencing primer:
T7
List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
Graham Warren at Yale, circa 2000
Principal Investigator:
Axel Brunger
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter
This plasmid has not been sequence verified. If you would like Addgene
to sequence a portion of this plasmid before you request it, please
contact us .
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
12356" in your Materials and Methods section.
