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pLPC myc hPOT1
(Plasmid #12387)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12387 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLPC
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Protection of Telomeres 1
  • Alt name
    POT1
  • Alt name
    2824
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1900
  • GenBank ID
    FLJ11073
  • Entrez Gene
    POT1 (a.k.a. CMM10, GLM9, HPOT1)
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TAGGCGTGTACGGTGGGA
  • 3′ sequencing primer CTTGCCAAACCTACAGGT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC myc hPOT1 was a gift from Titia de Lange (Addgene plasmid # 12387 ; http://n2t.net/addgene:12387 ; RRID:Addgene_12387)
  • For your References section:

    POT1 as a terminal transducer of TRF1 telomere length control. Loayza D, De Lange T. Nature. 2003 Jun 26. 423(6943):1013-8. 10.1038/nature01688 PubMed 12768206