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pAcGP67A - murine TfR
(Plasmid #12392)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 12392 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAcGP67A
  • Backbone manufacturer
    Pharmingen
  • Backbone size w/o insert (bp) 9761
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    transferrin receptor
  • Alt name
    TfR
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2000
  • Mutation
    See comments section
  • GenBank ID
    NM_011638
  • Entrez Gene
    Tfrc (a.k.a. 2610028K12Rik, CD71, E430033M20Rik, Mtvr1, TFR, TFR1, TR, Trfr, p90)
  • Tags / Fusion Proteins
    • leader peptide from baculovirus protein gp67 (N terminal on insert)
    • 6X-His tag (N terminal on insert)
    • Factor Xa cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EagI (not destroyed)
  • 5′ sequencing primer 5' AAT TTAA AAT gAT AAC CAT CTC gC 3'
  • 3′ sequencing primer 5' gAA gCg ggT CCA AgT TTC CCT g 3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gerhard Rank, PhD Rotary Bone Marrow Research Laboratories Royal Melbourne Hospital Royal Pde Parkville 3050 VIC Australia
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

The murine TfR sequence bp 367-2292 (of the ORF) was cloned into the pAcGP67A vector. The construct includes a short 3' UTR (past the stop codon at bp 2292). The TfR fragment was PCR amplified to introduce an EcoRI site at the 5' end and an EagI site at the 3' end. This was subsequently cloned into the corresponding EcoRI/EagI sites of pAcGP67A. The murine TfR was fused 3' to a gene segment encoding the hydrophobic leader peptide from the baculovirus protein gp67, a 6X-His tag, and a factor Xa cleavage site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcGP67A - murine TfR was a gift from Pamela Bjorkman (Addgene plasmid # 12392 ; http://n2t.net/addgene:12392 ; RRID:Addgene_12392)