OmpA-mNG-mCh
(Plasmid
#124216)
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PurposePeriplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mCh
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124216 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTHV037
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsAdd 0.5% glucose to repress expression
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameOmpA-mNG-mCh
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Alt namepNM004
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OmpA-mNG-mCh was a gift from Tanneke den Blaauwen (Addgene plasmid # 124216 ; http://n2t.net/addgene:124216 ; RRID:Addgene_124216) -
For your References section:
Activity-Related Conformational Changes in d,d-Carboxypeptidases Revealed by In Vivo Periplasmic Forster Resonance Energy Transfer Assay in Escherichia coli. Meiresonne NY, van der Ploeg R, Hink MA, den Blaauwen T. MBio. 2017 Sep 12;8(5). pii: mBio.01089-17. doi: 10.1128/mBio.01089-17. 10.1128/mBio.01089-17 PubMed 28900026
