MigR1-AE9a
(Plasmid #12433)

• Depositing Lab: Dong-Er Zhang
• Publication: Yan et al Nat Med. 2006 Aug . 12(8):945-9.

Backbone

• Vector backbone: MigR1
• Backbone manufacturer: See comments
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AML1-ETO9a fusion
• Alt name: AML1
• Alt name: ETO
• Species: H. sapiens (human)
• Mutation: AML-ETO protein with ETO exon 9a.
• Entrez Gene: RUNX1 (a.k.a. AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha)
• Entrez Gene: RUNX1T1 (a.k.a. AML1-MTG8, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: NA

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MigR1 is a MSCV-IRES-EGFP retroviral construct, similar to pMig (Addgene vector 9044). See "Pear, Baltimore, Blood. 1998 Nov 15;92(10):3780-92".

We used the oligonucleotides 5'-GTTTGGATGA TATGGCCATTG-3' and 5'-CCATCGATCCTCA TATGACCCAGGACAGC-3' in PCR with cDNA from Kasumi-1 cells to generate a DNA fragment from the BamHI site of ETO to the ETO9a coding sequence including the stop codon. We subcloned this fragment into the pGEM vector (Promega) and subsequently inserted it into the BamHI and EcoRI sites of MigR1-AE to form MigR1-AE9a.