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pCAGGS-FLAG-mISG15
(Plasmid #12443)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12443 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4790
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ISG15
  • Species
    M. musculus (mouse)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the ISG15 insert ends with the following amino acid sequence, LRLRGG. It is from pre-ISG15 which has a C-terminal deletion when compared to ISG15 sequences in GenBank. This plasmid is exactly as described in the associated publication listed below.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-FLAG-mISG15 was a gift from Dong-Er Zhang (Addgene plasmid # 12443 ; http://n2t.net/addgene:12443 ; RRID:Addgene_12443)
  • For your References section:

    Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for protein ISGylation. Kim KI, Giannakopoulos NV, Virgin HW, Zhang DE. Mol Cell Biol. 2004 Nov . 24(21):9592-600. 10.1128/MCB.24.21.9592-9600.2004 PubMed 15485925