nifF in pET21b
(Plasmid #124520)

• Purpose: Azotobacter vinelandii flavodoxin II of Nitrogenase
• Depositing Lab: Douglas Rees
• Publication: Segal et al Protein Sci. 2017 Oct;26(10):1984-1993. doi: 10.1002/pro.3236. Epub 2017 Aug 30.

Backbone

• Vector backbone: pET21b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5403
• Total vector size (bp): 5950
• Modifications to backbone: None
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use BL21 (DE3) for expression.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Flavodoxin II
• Alt name: nifF
• Species: Azotobacter vinelandii
• Insert Size (bp): 543
• GenBank ID:
• Entrez Gene: nifF (a.k.a. AvCA_01710)
• Promoter: T7 / lac
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Reverse

Resource Information

• Supplemental Documents:
  • niff-in-pet21b.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry