8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
(Plasmid #124543)

• Purpose: Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.
• Depositing Lab: Tudor Fulga
• Publication: Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi: 10.1038/s41467-019-09148-3.

Backbone

• Vector backbone: P1-EYFP-pA (Construct 5) (Addgene: 55197)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
• gRNA/shRNA sequence: Unknown
• Species: H. sapiens (human)
• Mutation: d11-41 C55G, d11-41
• Promoter: Minimal ADE

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Engineered tRNA scaffold-flanked sgRNA with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.

Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.