Addgene - pAd-RFP Plasmid Data

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12520: pAd-RFP
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Plasmid 12520: pAd-RFP

Gene/insert name:
RFP
Vector backbone:
pAdTrace-TO4
(Search Vector Database)
Vector type:
Mammalian Expression
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Roger Tsien, UCSD
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Tong-Chuan He
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

This is an RFP expressing adenoviral vector compatible with the AdEasy system. The mRFP is functional and the depositing lab has been using it as a control. The stop codon was originally deleted in order to make c-terminal fusion proteins. But the current construct should run into certain stop codons at the end of the linker sites.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12520" in your Materials and Methods section.