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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12559 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1(-)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5427
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCCAAT/Enhancer-binding Protein delta
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Alt nameC/EBP delta
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SpeciesM. musculus (mouse)
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Insert Size (bp)807
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MutationS2G introduced during cloning
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Entrez GeneCebpd (a.k.a. c/EBPdelta)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
When this C/EBPdelta construct was created, an NcoI restriction site was introduced that overlaps the start (AUG) codon to produce an optimal translation initiation site. This cloning strategy resulted in a S2G mutation in C/EBPdelta as a cloning "scar."
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA 3.1(-) mouse C/EBP delta was a gift from Peter Johnson (Addgene plasmid # 12559 ; http://n2t.net/addgene:12559 ; RRID:Addgene_12559)