pMSCV-TAP mLST8 (aka: GbL)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12575||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerSabatini Lab (Addgene Plasmid #12570)
- Backbone size w/o insert (bp) 6800
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)981
Entrez GeneMLST8 (a.k.a. GBL, GbetaL, LST8, POP3, WAT1)
/ Fusion Protein
- TAP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer MSCV rev primer (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
pMSCV-TAP was cut with XhoI/NotI and ligated to in-frame SalI/NotI inserts containing the sequences of mLST8.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSCV-TAP mLST8 (aka: GbL) was a gift from David Sabatini (Addgene plasmid # 12575 ; http://n2t.net/addgene:12575 ; RRID:Addgene_12575)
For your References section:mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, Sabatini DM. Curr Biol. 2006 Aug 15. ():. 10.1016/j.cub.2006.08.001 PubMed 16919458
Map uploaded by the depositor.