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pBabe hygro myc p110-CAAX
(Plasmid #12591)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12591 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe hygro
  • Backbone size w/o insert (bp) 5558
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p110-CAAX
  • Alt name
    PI3K
  • Alt name
    p110
  • Alt name
    PIK3CA
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    3300
  • GenBank ID
    NM_174574
  • Entrez Gene
    PIK3CA
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer pBabe 5'
  • 3′ sequencing primer pBabe 3'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Myc tagged p110-CAAX was cut out of pBabe-puro p110-CAAX using BamHI and ligated into BamHI cut pBabe-hygro.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe hygro myc p110-CAAX was a gift from Channing Der (Addgene plasmid # 12591)
  • For your References section:

    Oncogenic Ras blocks anoikis by activation of a novel effector pathway independent of phosphatidylinositol 3-kinase. McFall A, Ulku A, Lambert QT, Kusa A, Rogers-Graham K, Der CJ. Mol Cell Biol. 2001 Aug . 21(16):5488-99. 10.1128/MCB.21.16.5488-5499.2001 PubMed 11463831