pBabe hygro myc p110-CAAX
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12591||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBabe hygro
- Backbone size w/o insert (bp) 5558
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
SpeciesB. taurus (bovine)
Insert Size (bp)3300
/ Fusion Protein
- myc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer pBabe 5'
- 3′ sequencing primer pBabe 3' (Common Sequencing Primers)
Myc tagged p110-CAAX was cut out of pBabe-puro p110-CAAX using BamHI and ligated into BamHI cut pBabe-hygro.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe hygro myc p110-CAAX was a gift from Channing Der (Addgene plasmid # 12591)
For your References section:Oncogenic Ras blocks anoikis by activation of a novel effector pathway independent of phosphatidylinositol 3-kinase. McFall A, Ulku A, Lambert QT, Kusa A, Rogers-Graham K, Der CJ. Mol Cell Biol. 2001 Aug . 21(16):5488-99. 10.1128/MCB.21.16.5488-5499.2001 PubMed 11463831
Map uploaded by the depositor.