pBabe puro C-Raf 22W
(Plasmid #12593)

• Depositing Lab: Channing Der
• Publication: McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99.

Backbone

• Vector backbone: pBabe puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: N-term truncated mut of Raf-1
• Alt name: c-Raf 22W
• Alt name: Raf
• Species: H. sapiens (human)
• Mutation: 22W, N-terminally truncated
• Entrez Gene: RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBabe 5'
• 3′ sequencing primer: pBabe 3'

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry