pHRW-CRY2-VHH(GFP)
(Plasmid #126022)

• Purpose: express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogenetics
• Depositing Lab: Eurico Morais-de-Sá
• Publication: Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: biom9020061. doi: 10.3390/biom9020061.

Backbone

• Vector backbone: pHRW
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRY2 PHR-VHH(GFP)
• Species: A. thaliana (mustard weed)
• Mutation: WT
• Promoter: Hsp70
• Tag / Fusion Protein:
  • RFP (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CRY2 from Arabidopsis thaliana was obtained from pCMV-SNAP-CRY2-VHH(GFP) (Addgene 58370) as described in the methods of Osswald et al., 2019. CRY2olig-VHH(GFP) was generated by site-directed mutagenesis. Construct is heat shock inducible.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry