PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||126084||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2168
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)678
- Promoter J23150
- Cloning method Unknown
- 5′ sequencing primer GTTATTGTCTCATGAGCGGATAC (Common Sequencing Primers)
Cloning method was In-Fusion.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBbA11a-RFP was a gift from Nigel Scrutton (Addgene plasmid # 126084 ; http://n2t.net/addgene:126084 ; RRID:Addgene_126084)
For your References section:SelProm: A Queryable and Predictive Expression Vector Selection Tool for Escherichia coli. Jervis AJ, Carbonell P, Taylor S, Sung R, Dunstan MS, Robinson CJ, Breitling R, Takano E, Scrutton NS. ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00399. 10.1021/acssynbio.8b00399 PubMed 30870592