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CaMKIV-DCTK75E
(Plasmid #126423)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 126423 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6423
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    calcium/calmodulin-dependent protein kinase IV kinase-dead mutant
  • Alt name
    CAMK IV kinase-dead mutant
  • Alt name
    CAMK 4 kinase-dead mutant
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    989
  • Mutation
    changed lysine 75 to glutamic acid
  • GenBank ID
    NM_001744
  • Entrez Gene
    CAMK4 (a.k.a. CaMK IV, CaMK-GR, CaMKIV, caMK)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CaMKIV-DCTK75E was a gift from Douglas Black (Addgene plasmid # 126423 ; http://n2t.net/addgene:126423 ; RRID:Addgene_126423)
  • For your References section:

    A CaMK IV responsive RNA element mediates depolarization-induced alternative splicing of ion channels. Xie J, Black DL. Nature. 2001 Apr 19;410(6831):936-9. doi: 10.1038/35073593. 10.1038/35073593 PubMed 11309619