pcDNA3.1(-)-V5.mER.hOr47a
(Plasmid #126473)

• Purpose: Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells
• Depositing Lab: Bill Hansson
• Publication: Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5427
• Total vector size (bp): 6657
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Or47a
• Alt name: DmelOr47a
• Alt name: Odorant receptor 47a
• Species: D. melanogaster (fly)
• Insert Size (bp): 1236
• Mutation: codon optimization for H. sapiens
• Entrez Gene: Or47a (a.k.a. Dmel_CG13225, 47E.1, 47a, AN10, CG13225, DOR24, DOR47E.1, Dmel\CG13225, OR47a, Or24, Or47E.1, dor24, or47a)
• Promoter: CMV
• Tags / Fusion Proteins:
  • V5 tag (N terminal on insert)
  • H. sapiens HCN1 106VNKFSL111 (mER) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • pcDNA3.1(-)-V5.mER.hOr47a
  • V5.mER.hOr47a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint