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pCMVtight-UPRT-T2A-RFP-IRES-CD
(Plasmid #126677)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 126677 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti CMVtight Puro
  • Backbone manufacturer
    addgene
  • Backbone size w/o insert (bp) 7900
  • Total vector size (bp) 9900
  • Modifications to backbone
    cloned out eGFP
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UPRT-T2A-RFP-IRES-CD
  • Alt name
    uracil phosphoribosyltransferase
  • Alt name
    TGME49_312480
  • Alt name
    cytosine-deaminase
  • Species
    S. cerevisiae (budding yeast); Toxoplasma gondii
  • Insert Size (bp)
    2000
  • GenBank ID
    XP_002364470.1
  • Entrez Gene
    FCY1 (a.k.a. YPR062W)
  • Promoter tight TRE

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMVtight-UPRT-T2A-RFP-IRES-CD was a gift from Joan Massague (Addgene plasmid # 126677 ; http://n2t.net/addgene:126677 ; RRID:Addgene_126677)
  • For your References section:

    Flura-seq identifies organ-specific metabolic adaptations during early metastatic colonization. Basnet H, Tian L, Ganesh K, Huang YH, Macalinao DG, Brogi E, Finley LW, Massague J. Elife. 2019 Mar 26;8. pii: 43627. doi: 10.7554/eLife.43627. 10.7554/eLife.43627 PubMed 30912515