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Cav1-mRed
(Plasmid #12681)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12681 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    canine caveolin-1
  • Alt name
    cav1
  • Alt name
    VIP21
  • Species
    Canis familiaris
  • Insert Size (bp)
    1289
  • GenBank ID
    NM_001003296
  • Entrez Gene
    CAV1 (a.k.a. caveolin 1)
  • Tag / Fusion Protein
    • mRed (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    canine caveolin1 was cut from Cav1-GFP from Ari Helenius. Monomeric red (mRed or mRFP) was cut from a pRSETB plasmid from Roger Tsien, Howard Hughes Medical Institute
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Caveolin-Nat Cell Biol. 2001 May;3(5):473-83.

mRed-Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cav1-mRed was a gift from Richard Pagano (Addgene plasmid # 12681 ; http://n2t.net/addgene:12681 ; RRID:Addgene_12681)
  • For your References section:

    Selective stimulation of caveolar endocytosis by glycosphingolipids and cholesterol. Sharma DK, Brown JC, Choudhury A, Peterson TE, Holicky E, Marks DL, Simari R, Parton RG, Pagano RE. Mol Biol Cell. 2004 Jul . 15(7):3114-22. 10.1091/mbc.e04-03-0189 PubMed 15107466