pCS2-PLK1-mCherry
(Plasmid #127154)

• Purpose: Expresses mCherry PLK1 in mammalian cells and zebrafish embryos
• Depositing Lab: Heidi Hehnly
• Publication: Colicino et al Mol Biol Cell. 2019 Jun 15;30(13):1598-1609. doi: 10.1091/mbc.E18-12-0817. Epub 2019 May 1.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4091
• Total vector size (bp): 6619
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PLK1-mCherry
• Species: H. sapiens (human)
• Insert Size (bp): 2528
• Entrez Gene: PLK1 (a.k.a. PLK, STPK13)
• Promoter: CMV IE94
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cDNA sub-cloned from a vector used in Canton D. et al, Molecular Cell, Issue 4, 30 November 2012, Pages 547-559

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry