|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||127173||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepLenti (Addgene #86708)
Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter EF1a
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- 5′ sequencing primer tcttgtggaaaggacgaaaca
- 3′ sequencing primer aagcaccgactcggtgccac (Common Sequencing Primers)
Cloning Method: Golden Gate; 3' Cloning Site: aagcaccgactcggtgccac
Please visit https://www.biorxiv.org/content/10.1101/383943v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CROPseq-Guide-Zeo was a gift from Paul Blainey (Addgene plasmid # 127173 ; http://n2t.net/addgene:127173 ; RRID:Addgene_127173)
For your References section:Pooled optical screens in human cells. Feldman F, Singh A, Schmid-Burgk JL, Mezger A, Garrity AJ, Carlson RJ, Zhang F, Blainey PC. bioRxiv 383943 10.1101/383943