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pFUGW Cas9-BFP
(Plasmid #127396)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127396 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFUGW
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9
  • Insert Size (bp)
    5000
  • Promoter SVVF
  • Tag / Fusion Protein
    • BFP

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAGTCCTCCGACAGACTGAGTC
  • 3′ sequencing primer CATTAAAGCAGCGTATCCACATAGCG
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFUGW Cas9-BFP was a gift from Xiaowei Zhuang (Addgene plasmid # 127396 ; http://n2t.net/addgene:127396 ; RRID:Addgene_127396)
  • For your References section:

    Imaging-based pooled CRISPR screening reveals regulators of lncRNA localization. Wang C, Lu T, Emanuel G, Babcock HP, Zhuang X. Proc Natl Acad Sci U S A. 2019 May 28;116(22):10842-10851. doi: 10.1073/pnas.1903808116. Epub 2019 May 13. 10.1073/pnas.1903808116 PubMed 31085639