pLenti-mCherry-Mango II x 24
(Plasmid #127587)

• Purpose: Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter
• Depositing Lab: David Rueda
• Publication: Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi: 10.1038/s41467-020-14932-7.

Backbone

• Vector backbone: pLenti/pCW
• Backbone size w/o insert (bp): 7767
• Total vector size (bp): 9693
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry-Mango II x24
• Species: Synthetic
• Insert Size (bp): 1926
• Promoter: pTRE Tet ON
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: mCherry_F
• 3′ sequencing primer: Puro_R

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry