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PurposeOver-express mCherry-53BP1 fragment to monitor 53BP1 foci.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127658 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTRIP-CMV
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name53BP1
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SpeciesH. sapiens (human)
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MutationNucleotides 3658-5133 of TP53BP1 (NM_005657.2) fused to mCherry. Amino acids 1225-1716 for isoform 1.
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Entrez GeneTP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
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Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTRIP-CMV-mCherry-53BP1 was a gift from Nicolas Manel (Addgene plasmid # 127658 ; http://n2t.net/addgene:127658 ; RRID:Addgene_127658) -
For your References section:
The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus. Gentili M, Lahaye X, Nadalin F, Nader GPF, Puig Lombardi E, Herve S, De Silva NS, Rookhuizen DC, Zueva E, Goudot C, Maurin M, Bochnakian A, Amigorena S, Piel M, Fachinetti D, Londono-Vallejo A, Manel N. Cell Rep. 2019 Feb 26;26(9):2377-2393.e13. doi: 10.1016/j.celrep.2019.01.105. 10.1016/j.celrep.2019.01.105 PubMed 30811988
