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PurposeExpresses hCD19
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127889 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneMSCV
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Backbone manufacturerClonetech
- Backbone size w/o insert (bp) 5097
- Total vector size (bp) 6102
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehCD19
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SpeciesH. sapiens (human)
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Insert Size (bp)1005
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Mutationnone
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Entrez GeneCD19 (a.k.a. B4, CVID3)
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Tag
/ Fusion Protein
- no tags
Resource Information
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A portion of this plasmid was derived from a plasmid made byGot gene from NCBI
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Optimal packaging results are obtained using the pCL-10A1 Retrovirus Packaging Vector from Novus Biologicals.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-hCD19 was a gift from Anjana Rao (Addgene plasmid # 127889 ; http://n2t.net/addgene:127889 ; RRID:Addgene_127889) -
For your References section:
NR4A transcription factors limit CAR T cell function in solid tumours. Chen J, Lopez-Moyado IF, Seo H, Lio CJ, Hempleman LJ, Sekiya T, Yoshimura A, Scott-Browne JP, Rao A. Nature. 2019 Mar;567(7749):530-534. doi: 10.1038/s41586-019-0985-x. Epub 2019 Feb 27. 10.1038/s41586-019-0985-x PubMed 30814732
Map uploaded by the depositor.
