Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pCMS-dsRed2-WT LMNA-GFP minigene
(Plasmid #128230)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 128230 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMS-EGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6350
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lamin A
  • Alt name
    LMNA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    850
  • Entrez Gene
    LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • GFP (C terminal on backbone)
    • SV40-driven dsRed2 to identify transfected cells.

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Paula Scaffidi

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

WT lamin A splicing reporter with short-lived GFP downstream LMNA exon 12 (CMV-driven) + SV40-driven dsRed2 to identified transfected cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMS-dsRed2-WT LMNA-GFP minigene was a gift from Tom Misteli (Addgene plasmid # 128230 ; http://n2t.net/addgene:128230 ; RRID:Addgene_128230)
  • For your References section:

    Interplay of primary sequence, position and secondary RNA structure determines alternative splicing of LMNA in a pre-mature aging syndrome. Shilo A, Tosto FA, Rausch JW, Le Grice SFJ, Misteli T. Nucleic Acids Res. 2019 Apr 22. pii: 5475939. doi: 10.1093/nar/gkz259. 10.1093/nar/gkz259 PubMed 31006814