pX330GFP-hU6-gRNA-hSpCas9
(Plasmid
#128385)
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PurposeGFP positive gRNA/hSpCas9 construct
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128385 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepX330
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP, gRNA and hSpCas9
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byFeng Zhang, Broad Institute, [email protected]
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid was modified to include a GFP expression cassette.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pX330GFP-hU6-gRNA-hSpCas9 was a gift from Budd A. Tucker (Addgene plasmid # 128385 ; http://n2t.net/addgene:128385 ; RRID:Addgene_128385) -
For your References section:
CRISPR-Cas9-Based Genome Editing of Human Induced Pluripotent Stem Cells. Giacalone JC, Sharma TP, Burnight ER, Fingert JF, Mullins RF, Stone EM, Tucker BA. Curr Protoc Stem Cell Biol. 2018 Feb 28;44:5B.7.1-5B.7.22. doi: 10.1002/cpsc.46. 10.1002/cpsc.46 PubMed 29512106
