pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394
(Plasmid #128386)

• Purpose: Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption
• Depositing Lab: Craig Duvall
• Publication: Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acssensors.0c00103.

Backbone

• Vector backbone: pLV
• Backbone manufacturer: VectorBuilder
• Backbone size w/o insert (bp): 9096
• Total vector size (bp): 12473
• Modifications to backbone: EF1A promoter; EGFP:T2A:Bsd selection
• Vector type: Mammalian Expression, Lentiviral, Luciferase, Synthetic Biology
• Selectable markers: Blasticidin ; EGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: NLuc-3xGGGGS-Gal8-NCRD
• Alt name: Luc2
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1716
• Mutation: N terminal carbohydrate recognition domain fused to N terminal Luc fragment
• Entrez Gene: LGALS8 (a.k.a. Gal-8, PCTA-1, PCTA1, Po66-CBP)
• Promoter: EF1A
• Tag / Fusion Protein:
  • N-terminal luc2 fragment (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: none

Gene/Insert 2

• Gene/Insert name: Gal8-NCRD-3xGGGGS-CLuc398
• Species: H. sapiens (human)
• Insert Size (bp): 990
• Mutation: N terminal carbohydrate recognition domain fused to C terminal Luc fragment
• Entrez Gene: LGALS8 (a.k.a. Gal-8, PCTA-1, PCTA1, Po66-CBP)
• Promoter: IRES
• Tag / Fusion Protein:
  • C-terminal luc2 fragment (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: none

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Outsourced synthesis

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry