R4pGWB401+Citrine
(Plasmid #128432)

• Purpose: (Empty Backbone) Modified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.
• Depositing Lab: George Haughn
• Publication: McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.1007/s11103-019-00909-8. doi: 10.1007/s11103-019-00909-8.

Backbone

• Vector backbone: R4pGWB401
• Backbone manufacturer: Invitrogen modified by doi:10.1271/bbb.70678
• Backbone size (bp): 10835
• Modifications to backbone: The sequence encoding Citrine, a dimeric acid stable yellow fluorescence protein, was ligated downstream of the attR2 gateway site using the Eco47II and SacI sites, to enable C-terminal Citrine tagging during Three-way Gateway cloning.
• Vector type: Plant Expression ; Gateway destination vector
• Selectable markers: Kanamycin (35 ug/ml)
• Tag / Fusion Protein:
  • Citrine, yellow fluorescence protein (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: For selection use 100 ug/ml Spectinomycin
• Copy number: Low Copy

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: 5’-CGTCTGTTTGTGGATGTACAG-3
• 3′ sequencing primer: 5’-GGTGGTGCAGATGAACTTCAGG-3’

Resource Information

• Supplemental Documents:
  • R4pGWB401+Citrine - SnapGene File
• A portion of this plasmid was derived from a plasmid made by: Citrine sequence (768 bp) includes a N-terminal linker sequence (PGGGGGA) and a C-terminal linker sequence (DPAGAAAPLA) which was amplified from the pAD plasmid described in DeBono et al. 2011 (http://dx.doi.org/10.14288/1.0072411).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For use with Three-way Multi-site Gateway Cloning (Invitrogen).